Dante is a paranormal mercenary, private investigator and vigilante Devil Hunter dedicated to exterminating evil demons. Dante is incredibly flippant, casually mouthing off to even the most powerful of demons. He is the second son of the demon Sparda and the human Eva, the younger twin brother of Vergil, and the uncle of Nero.

Vergil is the older twin brother of Dante.Following the death of their mother as children, Vergil and Dante went their separate ways, with Vergil rejecting his humanity and embracing his demonic heritage. Stoic and reserved, Vergil displays a willingness to do anything in his quest to obtain the power of his father, Sparda.

He was raised in Fortuna and served as a Holy Knight in the Order of the Sword, a religious group that worships Sparda and fights to protect the world from demons. Nero was initially disgusted by his demonic abilities. He considered Devil Bringer is a curse and hid it out of shame. However, over time he began to train with the Devil Bringer until he finally embraces it as his power.

Lady is a human Devil Hunter. She originally hated all demons, even half-demons, and wanted to exterminate all of them. However, over time and through her experiences with Dante, she learns that humans and demons are equals in their capacity for both righteousness and depravity. While her preferred name, "Lady", was given to her by Dante, her birth name is Mary Ann Arkham.